Semrock has augmented its flagship BrightLine family of fluorescence filters with a new series of high performance fluorescence filter sets that are specially optimized for laser excitation, manufactured with hardcoated Ion-Beam Sputtering technology.

Semrock’s laser fluorescence filter sets (distribution Laser 2000) are crafted for the most popular lasers used in fluorescence imaging, including the newer all-solid-state lasers that are rapidly replacing older gas-laser technology. The new excitation filters offer excellent transmission at the designated laser wavelengths, combined with low ripple passbands. The separation of the excitation and emission filters is exceptionally small (less than 2.5% of the longest laser wavelength) to enable maximum capture of even weak signals close to the laser line. The matched dichroic beamsplitters exhibit excellent laser reflection for all polarizations, and transition rapidly to a wide and low-ripple fluorescence passband, with the flatness, low autofluorescence, and surface cosmetic properties needed for excellent imaging performance.

BrightLine Laser Fluorescence filter sets are designed for use in laser-scanning and spinning-disk confocal microscopes, as well as total-internal-reflection fluorescence (TIRF) microscopes. They are also ideal for any fluorescence microscope or imaging system that demands premium spectral performance and/or beamsplitter flatness, such as structured illumination systems for reduction of out-of-plane noise.

“Laser-based fluorescence microscopy is the fastest growing segment of the fluorescence imaging market, and customers are demanding the performance and reliability that Semrock can offer,” said Dr. Turan Erdogan, Semrock’s Chief Technology Officer.

Like all of Semrock’s optical filters, the new laser fluorescence filter sets are exclusively manufactured with hard-coated Ion-Beam Sputtering technology for Semrock’s famous “no burn-out” reliability, and they are confidently backed by an industry-leading five year warranty. These filter sets ship quickly from stock, as do an exceptionally diverse array of complementary fluorescence and laser-filter products.